Profile of drug resistance in Staphylococcus saprophyticus strains causing urinary tract infection in Women from Gorgan, Northern of Iran

Background and Aim : S.saprophyticus is a Coagulase-negative staphylococci that was reported in a ۱۹۶۲ Torres Pereira study to be associated with a urinary tract infection. Intrinsic resistance to novobiocin disc is a laboratory characteristic that distinguishes it from other species.The pathogenicity of S.saprophyticus is related to some major surface proteins that have been characterized as important for the pathogenesis and attachment to host tissues, and some enzymes, such as D-serine deaminase (DsdA), urease. Today, increasing antibiotic resistance has become a major challenge in the treatment sector. This study aimed to investigate the pattern of antibiotic resistance of S.saprophyticus isolates from urine samples in northern Iran (Gorgan)Methods : This study was performed from May ۲۰۱۸ to September ۲۰۲۰. The antibiotic susceptibility pattern of Staphylococcus saprophyticus isolates evaluation was carried out using the disc diffusion method (Kirby Bauer) according to (CLSI۲۰۱۹)Clinical and Laboratory Standards Institute. For identification of the isolates, ۱۶S rRNA gene were amplified. Some PCR products were sequenced and deposited in the GenBank.Results : Thirty-five isolates of S.saprophyticus had novobiocin disk resistance, Examination of growth in mannitol salt agar medium showed that ۲۲ isolates (۶۲.۹%) were able to ferment mannitol sugar. All isolates belonged to women (۱۰۰%) with urinary tract infections. Patients ranged in age from ۴ to ۶۵ years. (Mean ± SD) (۳۵ ± ۱۱/۸). The results of the antibiogram showed that All strains (۱۰۰%) were sensitive to the linezolid, Gentamicin, and Nitrofurantoin. the High sensitivity was also observed to Cotrimoxazole and Levofloxacin(۹۴/۳%). urinary tract infections caused by this bacterium have reported the existence of a seasonal distribution pattern. In the present study, ۲۴ isolates (۶۸.۶%) were related to the summer season.There is no clear reason for this seasonal variation.Conclusion : the choice of a solution to reduce resistance or use complementary or alternative therapies with antibiotics such as the use of bacteriophages in the treatment of urinary tract infections has been considered. The authors have also Isolation and Identification of S.saprophyticus Lytic bacteriophages from Urban and Hospital Wastewaters in Gorgan city، The results will be published soon.

کلیدواژه ها:

Urinary tract infection ، Staphylococcus saprophyticus ، Antibiotic resistance