Frequency of ACC, GES and PER genes in Acinetobacter baumannii clinical isolates by multiplex-PCR method

سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 219

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شناسه ملی سند علمی:

MEDISM22_072

تاریخ نمایه سازی: 8 مهر 1400

چکیده مقاله:

Background and Aim : Acinetobacter baumannii is the cause of a wide range of nosocomial infections. Antibiotic resistance of this organism is a major challenge worldwide. The aim of this study was to identify ACC, GES and PER genes and to determine the pattern of drug resistance.Methods : Antibiotic susceptibility testing was performed on ۲۵ bacterial isolates in plates collected from ICU samples using Müller-Hinton agar medium and disk diffusion method. MPprimer software was used to design the primers. After data collection, the level of significance was assessed at P˂۰.۰۵ using SPSS ۲۲.Results : There was a significant relationship between resistance pattern and sensitivity in all isolates with the type of antibiotic (except carbenicillin). All isolates (۱۰۰%) were sensitive to colistin Also, one isolate (۴%) had PER gene, ۴ isolates (۱۲%) had GES gene and ۷ isolates (۲۸%) had ACC gene.Conclusion : The results of this study indicate the identification of Acinetobacter baumannii isolates with high resistance to piperacillin, streptomycin, carbenicillin and aztronam. The abundance of resistance genes emphasizes the need for continuous monitoring to reduce the spread of resistance genes and prevent the emergence of resistant isolates, and this shows the necessity for more attention to health centers in prescribing appropriate drugs and avoiding the arbitrary consumption of antibiotics.

کلیدواژه ها:

Acinetobacter baumannii ، ACC ، GES and PER antibiotic resistance genes ، Multiplex-PCR ، Nosocomial infection.

نویسندگان

Reza Yari

Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran.

mohsen Mirzaei

Department of Laboratory Science, Borujerd Branch, Islamic Azad University, Borujerd, Iran

Mohammad Reza Mehrabi

Department of Laboratory Science, Borujerd Branch, Islamic Azad University, Borujerd, Iran

Maryam Valipoori

Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran.