The Link Between Specific Haplotype of Tumor Necrosis Factor Alpha and Interleukin-۱β Polymorphisms in Promoter Region with Helicobacter Pylori Infection and Gastric Carcinogenesis

سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 131

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شناسه ملی سند علمی:

MEDISM22_052

تاریخ نمایه سازی: 8 مهر 1400

چکیده مقاله:

Background and Aim : The infection with Helicobacter pylori (H. pylori) and cytokine-mediated inflammatory responses plays significant roles in the pathogenesis of gastric cancer (GC). To determine a link between the risks of GC and genetic polymorphisms in tumor necrosis factor-alpha (TNF-α), and interleukin (IL)-۱β, this study was performed.Methods : The polymorphisms of IL۱B and TNFA genes were analyzed by PCR-RFLP in ۲۹۰ patients who underwent endoscopy. H. pylori infection was affirmed by rapid urease test, histological analysis, and gastric biopsy PCR. The quantitative real-time PCR was performed to determine the relative mRNA expression levels. Results : There were no significant differences in allele frequencies and genotype for all explored polymorphisms between chronic gastritis, GC and healthy individuals. Interleukin ۱β mRNA was down-regulated in both the gastritis group (Relative Quantification (RQ)=۰.۴۴۷) and the GC group (RQ=۰.۱۵۱). In contrast, the expression of TNF-α were increased in the GC group (RQ=۲.۸۱۷) in relation to the gastritis group, which was down-regulated (RQ=۰.۸۶۱). Conclusion : The studied single-nucleotide polymorphisms are not risk factors for development of chronic gastritis and GC. However, the H. pylori infection causes a huge increase in the expression of TNF-α in GC patients.

نویسندگان

Aylin Esmailkhani

Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Abed Zahedi bialvaei

Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Fatemeh Nezamzadeh

School of Medicine, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran