Facile green synthesis and characterization of Pistacia khinjuk leaves extract capped silver nanoparticles (PKL@AgNPs): Antibacterial, antifungal, anti-cancer, antioxidant and catalytic activities

Background and Aim : In recent years, green nanoparticles have found many applications especially as alternative antimicrobial approaches due to their unique physicochemical, biological, mechanical, optical, electrical and thermal properties characterized by their small size and high surface-to-volume ratio. This study aimed to synthesis a green capped silver nanoparticle using Pistacia khinjuk leaves and evaluate its antimicrobial, antioxidant, anticancer and catalytic activities. Methods : In this study for the first time, biogenic silver nanoparticles were synthesized using nontoxic, low-cost and rapid approach in presence of Pistacia khinjuk leaves extract (P. khinjuk) as PKL@AgNPs. The biosynthesized AgNPs were characterized in terms crystalline, morphology and structural with XRD, EDS, FESEM, TEM, FT-IR, DLS and UV-Vis techniques. Antibacterial and antifungal activities of biosynthesized AgNPs were investigated using MIC, MBC and MFC. The antioxidant activity of PKL@AgNPs was calculated by scavenging free radicals of DPPH (۱,۱-diphenyl-۲-picrylhydrazyl hydrate). Anticancer activity was evaluated on k۵۶۲ as a human leukemia cancer cell line by MTT assay. To assess its catalytic feature, photocatalytic activity for the degradation of the methylene blue (MB) and methyl orange (MO) as hazardous contaminants was applied.Results : FESEM, TEM and XRD analysis results revealed that the product was homogeneous, uniformly, spherical-like morphology regular and small size (about ۳۵-۴۵ nm) with face-centered cubic structure. PKL@AgNPs showed strong antibacterial and antifungal activities against Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Streptococcus mutans, Streptococcus mitis, Enterocuccus faecalis, and Candida albicans. The antioxidant results revealed that with increasing the concentration of nanoparticles, the percentage of DPPH inhibition increases (۳.۸% to ۶۹.۹%). The PKL@AgNPs also revealed significant anticancer activity on k۵۶۲ (IC۵۰ = ۲.۶۹ µg/ml). Furthermore, the biosynthesized AgNPs exhibited a high photocatalytic activity for the degradation of MB and MO.Conclusion : PKL@AgNPs as a novel facile green synthesized nanoparticle by Pistacia khinjuk leaves has highly significant antimicrobial, anti-cancer, anti-oxidant and catalytic activity which can be in medicine and biotechnology industry.

کلیدواژه ها:

Silver nanoparticles ، Antibacterial ، Antifungal ، Anticancer ، Pistacia khinjuk