Investigation of the presence of OmpL۱, LipL۴۱ proteins’ in the outer membrane of Leptospira

Background and Aim : Leptospirosis is zoonotic disease which caused by pathogenic leptospires in humans. The disease is one of the most common diseases in humans and animals. The outer membrane of leptospira contains lipopolysaccharide and several lipoproteins known as OMP, which play an important role in the pathogenesis process. The aim of this study was to investigate the presence of LipL۴۱ and OmpL۱ proteins Methods : The sequences used for cloning and expression were based on the serovars in the collection of Razi Serum and Vaccine Institute and were designed using snapgen software. The construct was prepared based on the pathogenic serovars. The target construct was then transferred into Ecoli PlysS bacteria and the expressed protein was confirmed using SDS page test. Results : In total, the presence of LipL۴۱ and Ompl۱ in the existing serovars was well cloned and expressed and confirmed by SDS page test with clear wind at ۴۳ kDa. Conclusion : This study shows that the recombinant antigen produced may be used in the future as a recombinant vaccine as well as the design of an ELISA kit for diagnosis.

کلیدواژه ها:

Leptospira ، SDS page ، OmpL۱ ، LipL۴۱