Cell substrates play a crucial role in tissue engineering and biomaterial science. Various studies were conducted to develop the appropriate cell substrates for useing in vitro and in vivo. However, it is not easy to provide a biocompatible substrate that mimics the native extracellular matrix properties for cells. Therefore, the substrate with the specified surface topography as a biomimicry factor and ideal physicochemical properties is necessary under ''the novel cell substrates development'' approaches. Our aim in the current study was to design, synthesize, and characterize a substrate with aligned nanometric wavy arrays on the surface. The rapid and easy capabilities of
Polydimethylsiloxane to receive chemical, mechanical, and physical characteristics with simple modifications, make it a promised candidate for the cell substrate. The obtained results from the atomic force microscope showed that thewrinkled nanoarrays were formed on the surface after regulating the substrate under lateral traction during the plasma treatment. With increasing the plasma treatment time from ۱۰۰ to ۲۰۰s, both wavelength and amplitude of our structures were raised by ۸۷ and ۱۴۲%, respectively. Then, the behavior of HU۰۲, a human foreskin fibroblast cell line, in terms of adhesion, growth, viability, and morphology on this substrate was investigated. The more increase the plasma treatment time, the more increase of surfaces hydrophilicity, lead to significantly improve the quality of cell attachment. Additionally, the presence of the designed nanowrinkles surprisingly improved the number of the attached cells. This improvement was from ۵۲.۲% of the seeded cells to۷۴.۹% for substrates with a plasma treatment time of ۱۰۰s, while in ۲۰۰s of plasma treatment it was from ۶۶.۸ to ۷۸.۰%. The nanowrinkles caused the cells to align perfectly through the substrate's surface with a standard deviation of ۱۱.۴۹° from the average alignment angle.