The antifungal effect of thionin-like peptide (ThiL۲۰) against pathogenic fungus of Botrytis cinerea

Botrytis cinerea is one of the most important plant pathogen fungi in most regions of the world. In this study, the antifungal activity of purified plant peptide of thionin-like (ThiL۲۰) was investigated with different concentrations against main species of Botrytis. Thionin-like gene (At۲g۲۰۶۱۸) was isolate from Arabidopsis and cloned in E. coli (strain C۳۰۳۰) carrying pETtrx۱-a vector. The antifungal peptide was purified using reverse-phase high-performance liquid chromatography (RP-HPLC). The results of antifungal tests by adding to liquid broth culture with different concentrations using in vitro bioassay shown that the ThiL۲۰ peptide had high antifungal activity and inhibitory effect and in ۱۰۰ and ۲۰۰ μg/ml had inhibitory effect completely against Botrytis cinerea species in comparison to control.

کلیدواژه ها:

inhibitory effect ، plant antimicrobial protein