Evaluation of antifungal activity of Rydingia michauxii extracts at the three different growth stages

Rydingia michauxii (Lamiaceae) is an endemic herb to Fars province, Iran, traditionally employed for treating a wide number of human diseases. In the present study, the antifungal activity of R. michauxii methanolic extracts at the dormant, vegetative and flowering stages was evaluated on a number of fungi (Rhizoctonia solani, Bipolaris micropus, Fusarium solani and Fusarium oxysporum). The antifungal activity of R. michauxii extracts was assessed by the minimum inhibitory concentration (MIC) method according to p-iodonitrotetrazolium chloride assay. The MIC was recorded as the first concentration of the plant extract with no color change of the medium showing no bacterial growth. The average MICs displayed by the R. michauxii extracts showed that R. solani and F. oxysporum had the most sensitivity (۱۸.۶۷ mg/ml) and resistance (۲۴ mg/ml) to the extracts, respectively. It seems that differences in content and amount of compounds of R. michauxii extracts at the various growth stages are responsible for the level of antifungal activity.

کلیدواژه ها:

Fungi ، Minimum inhibitory concentration ، Plant extract ، Rydingia michauxii