Investigation of Genetic Diversity of Quercus brantii Lindl. Populations Using ISJ Semi-random Markers

The evaluation genetic variations in trees has important results for the conservation of genetic resources, and the use of molecular markers is the best way to identify genetic diversity. In this study, for the first time, genetic diversity within and between populations of ۱۰ populations from Persian oak species (Quercus brantii Lindl.) was investigated using ISJ (Interon-exon Splice Junction) molecular markers in Lorestan province. In this study, ۵۰ samples from different bases of Persian oak species and ۱۰ ISJ primers including IT and ET primers with different nucleotide numbers were used (Panjoo et al., ۲۰۱۴). The molecular variance (AMOVA) showed that genetic diversity within populations was ۷۶% higher than between population diversity at ۲۴% using GenALEx ۶.۵. The values of genetic differentiation coefficient (FST) and gene flow (Nm) were ۰.۱۱۴ and ۱.۹۳۸, respectively. Also, the initiators created ۱۹۱۱ bands, that about ۱۸۹۳ were polymorphic bands. The highest number of polymorphic bands was related to IT ۱۰-۳ primer pairs, IT ۱۰-۶ with ۵۲۳ bands and the lowest number of polymorphic bands was related to IT ۱۰-۱, IT ۱۰-۳ primer pairs with ۴۲۸ bands. Also, the highest and lowest amount of polymorphic information (PIC) is related to the pairs of primers ET ۱۵-۳۴, ET ۱۵-۳۵ about ۰.۹۵ and ET ۱۵-۳۳, ET ۱۵-۳۴ about ۰.۹۰, respectively. The dendrograms of Persian oak populations were plotted using the Jaccard similarity coefficient based on the UPGMA method in NTSYSpc ۲.۱۰e. Individuals of Persian oak species were clustered in two main groups, that sample number four of Kakareza population in a group and other samples from other populations in another group. In general, it can be concluded that the use of polymerase chain reaction using semi-random ISJ markers to separate different sampels from different populations of Persian oak species is appropriate.

کلیدواژه ها:

Primer ، Persian Oak ، Molecular Markers ، Interon-exon Splice Junction