Study of genus Ceratophyllum L. based on morphological, anatomical and molecular marker features in Iran

The genus Ceratophyllum belongs to Ceratophyllales and Ceratophyllaceae. Two species of this genus have been reported in the Iranian plateau. This genus is an aquatic submerged plant. Regarding the importance of Ceratophyllum due to pharmaceutical and bioenvironmental usages and the value of understanding species diversity, in this study species of this genus was examined by morphological, anatomical and molecular characters. For this purpose, sampling were done from two species of this genus including ۱۹ poulations of C. demersum with distribution in throughout of Iran (north, northwest, center and southeast) and ۴ populations of C. submersum with distribution in north and southwest of country. Twenty six quantitative and qualitative morphological characters were selected and a dendrogram was drowned. Cross section of leaves and stems were stained and observed by light microscope. The morphological characters of flowers, fruits and leaves and anatomical characters such as central parenchyma in the stems and number of aerenchyma in leaves demonstrated higher variation in different populations of two species. Also for genetic diversity of different populations, ۱۰ SRAP primers were used. The molecular results showed higher genetic variation within populations than between populations and central populations were separated from other populations. Different used characters in this study were important for identification and delimitation of species in this genus

کلیدواژه ها:

Horn leaf ، Morphology ، Intraspecific Diversity ، Ceratophyllaceae ، SRAP