Investigation of phytochemical diversity of Daphne mucronata collected from three regions of West Azerbaijan province

Daphne mucronata is a shrub belonging to the Thymelaeaceae family that is known as a medicinal plant in various parts of Asia. Daphne flowers, leaves and fruits are a rich source of important natural compounds, which are used to various diseases such as cancer, rheumatism, arthritis and muscle swelling. Geographical distribution of this species has been reported in several regions of Iran. The aim of this study was to investigate the phytochemical compounds in flowers, leaves and fruits of three samples collected from Mirabad, Qaleh Rash and Dige regions located in Sardasht, West Azerbaijan province. The results showed that there was a significant difference between samples collected from different regions for total phenol of flower (۰.۹۱-۱.۶۳ mg GAE g-۱ DW), total phenol of leaf (۰.۴۳- ۰.۹۷ mg GAE g-۱ DW), total phenol of fruit (۱.۷۸-۱.۹۱ mg GAE g-۱ DW), total flavonoid of flower (۰.۱۲-۰.۴۲ mg QE g-۱ DW), total flavonoid of leaf (۰.۰۰۳۲-۰.۰۰۴۵ mg QE g-۱ DW), total flavonoid of fruit (۰.۱۳۲-۰.۳۲۳ mg QE g-۱ DW), antioxidant activity of flower (۷۲.۴۵-۸۸.۲۸%), antioxidant activity of leaf (۵۲.۰۶-۷۷.۹%) and antioxidant activity of fruit (۶۰.۶-۶۷.۶۴%) by DPPH method. The highest amount of total flower and fruit phenol, total flower and leaf flavonoids and fruit antioxidant activity in Mirabad sample, the highest amount of total leaf phenol, total fruit flavonoids and leaf antioxidant activity in Qaleh Rash sample and the highest amount of flower antioxidant activity were observed in Dige sample. The leaf samples had lower total phenols and flavonoids than flower and fruit samples. Overall, the findings of this study showed that there is a significant phytochemical diversity between the samples collected from different regions.

کلیدواژه ها:

Antioxidant activity ، Daphne mucronata ، Total flavonoid ، Total phenol