Isolation and identification of secondary methabolites Using GC-MS analysis of chloroform-methanolic extracts of fruits in Heracleum persicum Desf Ex Fischer

Golpar, Heracleum persicum Desf Ex Fischer (Apiaceae) is one of important medicinal plants which to Iran. The fruits of this plant are widely used as spices and flavorings agents in foods as well as traditional herbal medicines for soothing, disinfecting, improving digestion and analgesia. In this study, in order to investigate the natural variation in the level of secondary metabolites was investigated quantitatively and qualitatively using GC-MS analysis of chloroform-methanolic extract of fruits from ۷ populations in Tehran (Tangeh-savashi, Shemshak), Ardabil (Anbaran), West Azerbaijan (Movana, Rezi(Ghotor), Silvana) and East Azerbaijan (Sambran). A total of ۹۷ compounds were identified in all population, in which ۱۱ compounds including γ-sitosterol (۰.۸ - ۷.۸۶), squalene (۰.۲۹ - ۰.۸۲), oleic acid (۱.۶۱ - ۴.۱۰), pimpinellin (۳.۹۳ - ۱۴.۵۹), hexadecanoic acid (۰.۸۲ - ۷.۷۱), n-octyl ۲-methylbutyrate (۲.۲۳ - ۷.۹۵), hexyl hexanoate (۲.۱۴ - ۵.۴۶), octyl ۲-methylpropanoate (۱.۲۷ - ۹.۴۴), pentylcyclopropane (۱۱.۰۱ - ۱۸.۹۸), hexyl butyrate (۱۹.۰۳ - ۳۳.۷۱), ۲-methylhexyl propanoate (۱.۶۸ - ۳.۸۱) were commonly reported in all populations. The amount of major compounds varied from ۶۴ to ۸۰% in Movana and Tangeh-savashi respectively. Although the content and amount of secondary metabolites identified with chloroform-methanolic extract is different from that detected with distillation method, But hexyl butyrate and several others common compounds is detected at high level in both methods. The results show that Heracleum fruits have might have medicinal and cosmetic values. Some of variation in the level of compounds among populations could probably be explained by environment conditions.

کلیدواژه ها:

golpar Heracleum ، chloroform-methanolic extract ، GC-MS ، secondary metabolites