Evaluation of Cytomegalo, Epstein-Barr and Varicella-Zoster viruses in Leukemias by PCR

Leukemia, as a complex disease, causes molecular changes in the cell by multiple variables. This disease interferes with the proliferation of bone marrow stem cells. It is classified in acute to chronic categories based on the invasion degree and its prevalence is increasing in our country. Therefore, identification of viral risk factors is very important, including CMV, EBV and VZV, all of which are the members of Herpesviridae family and have the potential for reactivation. For example, CMV is observed from prenatal to post-puberty, EBV with infection in B lymphocytes and VZV with dermal spread. Since the etiology of leukemia is unknown, we decided to evaluate these three viruses in types of leukemia and healthy controls using PCR. From ۱۰۰ blood serum samples of leukemia patients and ۱۰۰ healthy control samples prepared from Tehran laboratories, DNA was extracted from phenol-chloroform method from samples and All three optimal PCR tests and CMV, EBV and VZV amplicons with ۲۵۷ bp, ۱۲۹ bp and ۲۱۶ bp respectively were observed by agarose gel electrophoresis method. In the feature test of all three tests, only the target viral patterns responded positively. The detection limit was ۱۰۰ Copy / Reaction for CMV and ۱۰ Copy / Reaction for EBV and VZV. All healthy controls were negative, ۹% of the samples were infected with VZV, ۳۳% were infected with CMV and ۳۶% were infected with EBV.Infectious agents in leukemias are controversial today, and these three viruses, can be possible causes of leukemias

کلیدواژه ها:

Specificity test ، Etiology ، Amplicon