Evaluation of antioxidant activity and toxicity of LFcin-۱۱ peptide on gastric cancer cell biosynthesis (AGS)

Oxidative stress is the result of increased production of reactive oxygen species, which causes various diseases, including cancer. Antioxidant enzymes, as the body's defense system, reduce reactive oxygen species. In this study, peptide fragment containing ۱۱ amino acids (LFcin-۱۱) derived from Lactoferricin, which has a functional Lactoferricin sequence, was chemically synthesized. Then, antioxidant activity, toxicity and anti-cancer effect on AGS cells were investigated. The effect of peptide toxicity on gastric cancer cells was investigated by MTT assay. The results of the MTT test show a direct relationship between the concentration of the synthesized peptide and its lethal effect on gastric cancer cells. Also, the results of measuring the antioxidant activity of LFcin-۱۱ peptide indicate the ability of the peptide to remove DPPH radicals. Due to its antioxidant activity and anti-cancer properties of LFcin-۱۱ peptide, this peptide can be used in the treatment of cancer.

کلیدواژه ها:

Reactive oxygen species ، Lactoferricin ، Antioxidant ، Cell Viability