Comparison of Pentose Phosphate Metabolism Pathway Genes Expression in Aerobic and Anaerobic Conditions in Wild Strain and Alcohol Deheydrogenase Negative Escherichia coli

Escherichia coli is widely used in the biotechnology industry and in the production of various pharmaceutical products and has the ability to grow well in fermenters and other manufacturing devices under various aerobic and anaerobic conditions. Metabolic engineering is a new way to achieve mass production and high efficiency in Is the science of biotechnology.One of the methods of metabolic engineering is to pay attention to the expression of the desired bacterial gene in different growth conditions and to identify important genes that regulate the metabolic pathway. The metabolic pathway of pentose phosphate is an early and important pathway that has existed in prokaryotic and eukaryotic cells since the beginning of life. Evaluation of phosphate pathogen-producing genes in Escherichia coli under different aerobic and anaerobic conditions in mutant and natural species; The aim of this research is to achieve high product production in Fermentor. To conduct this research, all enzymes of the pentose phosphate pathway in the target bacterium have been identified through the KEGG database of enzymatic components and scientific characteristics (such as enzyme number, gene locus, homologous and orthological information). In the next step, GSE۴۶۴۵۵ and GSE۱۱۲۱ microarrays, which are related to the expression of the genes of this bacterium in aerobic and anaerobic conditions, were identified in both mutant and natural mutations on the NCBI website. Then, through the meta-analysis method in the GEO section of the NCBI website, we compared different microarrays in terms of the expression of the identified genes (the basis for comparing the two criteria logFC and Pvalue is the graph of each gene). Depending on aerobic, anaerobic, mutant and natural species; There are ۴ modes for each microbial. The results of each microbial study showed that the gene mutation reduces the expression of pentose phosphate genes in aerobic and anaerobic conditions. The highest amount of gene expression is related to anaerobic conditions and the mutant species reduces the expression several times more than the normal species. Expression of glucose-۶-phosphate dehydrogenase genes; increased ۶-phosphogluconate dehydrogenase and gluconokinase genes; Transaldolase and deoxyribose-phosphate aldolase have shown a decrease in gene expression, so it can be concluded that the expression of genes in the metabolic pathway of pentose phosphate, especially oxidative genes, is highest in anaerobic conditions in the natural strain. It can be said that bacteria use this pathway to produce regenerative power and power generation to deal with stress and lack of oxygen.

کلیدواژه ها:

Meta-analysis ، Microarray ، GSE۴۶۴۵۵ ، GSE۱۱۲۱ GEO۲R