Synthesis and Characterization of ۵-Fluorouracil-loaded Calcium Carbonate Nanoparticles and their Cytotoxicity on Colorectal Cancer Cells

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 215

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شناسه ملی سند علمی:

BIOCONF21_0792

تاریخ نمایه سازی: 7 شهریور 1400

چکیده مقاله:

Colorectal cancer (CRC), as the third most prevalent cancer and fourth principal cause of cancer death worldwide, is in urgent need of effective treatments. Though chemotherapy is still one of the powerful tools available for cancer therapy, it suffers from some limitations including the lack of selectivity, aggregation, and low biocompatibility. Therefore, the application of targeted drug delivery systems (DDS) is of greatest importance, among which nanoparticles have attracted considerable attention as carriers. In this study, after Calcium Carbonate (CaCO۳) nanoparticles were synthesized via spontaneous precipitation method, the synthesized nanoparticles were characterized by X-ray Diffraction (XRD), Fourier transform infrared (FT-IR), and Field Emission Scanning Electron Microscope (FE-SEM). ۵-fluorouracil (۵-FU), a common anti CRC therapeutic drug, was loaded on CaCO۳ nanoparticles. Then, the cytotoxicity of ۵-FU alone and together with CaCO۳ nanoparticles were evaluated on the murine colorectal cell line, CT-۲۶, by MTT assay. The successful synthesis of CaCO۳ nanoparticles was concluded owing to the crystallinity, purity of CaCO۳ nanoparticles and the appearance of the characteristic vibrational bands attributed to the bending and stretching vibrations of CO۳۲- in XRD pattern and FTIR spectrum respectively. Furthermore, after ۵-FU loading on CaCO۳ nanoparticles, absorption bands which belongs to this drug emerged in its FTIR spectrum. Additionally, FE-SEM observations revealed the synthesis of oval-shaped CaCO۳ nanoparticles. The examination of the cytotoxicity of the drug-loaded nanoparticles on cancer cells showed that although free ۵-FU has inhibitory effects on cancer cells with the IC۵۰ value of ۱.۹±۰.۱ μg ml-۱, the chemotherapeutic drug efficacy is improved when loaded on CaCO۳ nanoparticles (IC۵۰ = ۰.۹±۰.۱۶ μg ml-۱). Based on the findings of this study, ۵-FU-loaded CaCO۳ nanoparticles could be considered as a promising candidate for colorectal cancer therapy.

نویسندگان

Mehrnaz Jahedi Moghaddam

Master's student, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad

Azadeh Meshkini

Associate Professor, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad