Fecal carriage of carbapenem resistance genes bla OXA-۴۸ and bla NDM-۱ in members of Enterobacteriaceae among hospitalized immunosuppressed children in the oncology ward of Mofid Children's Hospital in Tehran, Iran

In the last few years, the frequency of carbapenem resistance genes among Enterobacteriaceae have been increasing through the world, which most of them are the cause of hospital and society acquired infections and mortality especially in high risk infection patients such as immunosuppressed patients. This study investigates the colonization and frequency of blaOXA-۴۸ and blaNDM-۱ genes as main reasons of carbapenem resistance, in carbapenem resistance Enterobacteriaceae separated from the intestinal tract of immunosuppressed children admitted to the oncology ward of Mofid Children's Hospital in Tehran during ۶ months.Stool samples are collected during the first ۲۴ hours of hospital admission, then Enterobacteriaceae isolates recognized with standard microbiological laboratory methods, carbapenem resistance isolates identified by using antibiogram test and the intended resistance genes recognized buy PCR and electrophoresis gel. Frequency of isolates were like: ۲۶ Eschershia, (۵۷.۷%); ۱۰ Klebsiella, (۲۲.۲%); ۵ Enterobacter, (۱۱.۱%); ۲ Citrobacter, (۴.۴%) and ۱ Serrati (۲.۲), based on antibiogram tests, ۱۹.۲% Eschershia (۵/۲۶), ۴۰% Klebsiella (۴/۱۰), ۲۰% Enterobacter (۱/۵) and ۵۰% Serrati (۱/۲) had identified, which all ۱۲ of them had blaOXA-۴۸ and ۵ of them had blaNDM-۱.The conclusion of this study showed the high existence of carbapenem resistance isolates in oncology ward. The high existence of blaOXA-۴۸ in these isolates, especially in Escherichia, can raise therapeutic challenges and concerns in case of systemic infections in these patients.