Detection of Tomato spot wilt virus (TSWV) from Pelargonium hortorum in Hamedan city

Tomato spotted wilt virus (TSWV) is an Orthotospovirus of the family Tospoviridae, first reported in Australia in ۱۹۱۹ and causing extensive damage to vegetables, legumes and ornamental species. This virus has the worldwide spread and is one of the ten most harmful plant viruses in the world. TSWV is transmitted by thrips in a circulatory and propagative manner in nature. The virion of the virus is isometric and has a three-part genome in the form of single-stranded RNA. In order to detect the virus, ۳۵ samples of Pelargonium, chrysanthemum and Dahlia flowers were collected from Hamedan city, which showed symptoms of suspected infection such as yellowness, chlorosis and necrosis spots, deformation and wilting. The extract of suspicious plants was mechanically inoculated on indicator plants including beans, cucumbers and Chenopodium quinoa. After about two weeks, in indicator plants which inoculated with Pelargonium extract, symptoms such as necrosis and deformation appeared in bean leaves, as well as chlorotic spots in cucumber and Chenopodium leaves. Then, total RNA was extracted from symptomatic indicator plants and the contamination of the samples was confirmed using RT-PCR test and specific primers of TSWV coat protein gene. This is the first report of TSWV infection of Pelargonium flowers in Hamedan city. The virus was reported in ۲۰۰۰ by Moeini and Izadpanah from a Pelargonium in Tehran. Also in the same year, it was separated and reported from the city of Mahallat in Markazi province by Hassani Mehraban and Shahraein from Pelargonium. The best way to control viral diseases is to prevent the occurrence and spread of infection. Therefore, considering that the method of propagation of most ornamental plants is through vegetation, creating virus-free and safe seedlings, onions and vegetative parts is one of the effective ways to prevent the spread of viral diseases in ornamental plants.

کلیدواژه ها:

Identification ، Molecular Method ، Pelargonium