Detection of Potato Virus Y (PVY) from Solanum alatum weed in the Potato Fields of Hamedan Province

Viral diseases are among the factors limiting potato production in the world. Potato virus Y (PVY) from the family Potyviridae is one of the most important plant pathogens in the world, which reduces the quality and yield of Solanaceae family products such as tobacco, potatoes, tomatoes, peppers and weeds of this family and its worldwide spread is an ongoing challenge. PVY has a positive single- stranded RNA and its molecular weight is about ۹.۸ kb. The virus is transmitted mechanically as well as by the green peach aphid Myzus persicae. In order to detect the virus, ۳۰ samples of Solanum alatum that showed suspected signs such as mosaic, wavy leaf margin, chlorotic spots and leaf deformation were collected from Bahar city of Hamedan. Suspicious plant extracts were inoculated on indicator plants including Nicotiana occidentalis and N. glutinosa. After one week, signs of necrosis, vein clearing and mosaic were systematically observed in inoculated plants. Total RNA was extracted from symptomatic indicator plants and PVY contamination of samples was confirmed using RT-PCR test and specific primers of PVY coat protein gene. This is the first report of Solanum alatum infection to PVY in Hamedan province. This virus was reported in ۲۰۰۸ by Shamseddin Saeed et al. from Solanum alatum, Chenopodium urbicum, Emex spinosus and Physalis divaricata in Jiroft, and from Geranium pusillum and Lactuca serriola weeds in ۲۰۱۰ by Pourrahim et al. Because weeds are winter hosts of plant viruses, early detection of virus-infected weeds plays an important role in disease control and epidemiology.

کلیدواژه ها:

Identification ، Molecular Method ، Solanum alatum