Biosynthesis and characterization of silver nanoparticles synthetized using extract of Haplophyllum obtusifolium and evaluation of the anti-cancer properties of synthesized nanoparticles

Silver nanoparticles exhibit unusual physicochemical properties and biological activity. With extensive research activities, the use of silver nanoparticles, especially in the field of health, has become widespread. Scientists are using biological methods (green methods) as environmentally friendly and cost-effective ways to synthesize nanoparticles and nanomaterials. In this study, the biological approach was aimed at producing nanoparticles from Haplophyllum obtusifolium watery extract. The biochemical nature of the plant extract was investigated and analyzed to determine the total amount of phenolic and flavonoid compounds with spectrophotometry. Several methods have been used to identify synthesized nanoparticles, the most important of which are: transverse electron microscopy (TEM), Ultraviolet–visible spectroscopy, Fourier-transform infrared spectroscopy )FTIR) and X-ray diffraction spectroscopy (XRD) which indicates the shape and structure of the nanoparticle. In this study, the toxicity of silver nanoparticles synthesized against the two major categories of Mcf۷ and HepG۲ cancer cells was extensively investigated by using MTT assay method, which strongly confirms the toxicity of nanoparticles against cancer cells. New advances in nanobiotechnology are a clear indication of the potential for cancer treatment.

کلیدواژه ها:

Green method ، Morphology ، Metastasis ، Apoptosis ، Angiogenesis