Expression and Diagnostic Values of Long Non-Coding RNAs, MIAT, H۱۹, and NRON, in Multiple Sclerosis Patients

Multiple sclerosis (MS) is a chronic complex autoimmune disease of the CNS. The specific criteria for MS are axonal degeneration and demyelination. Long non-coding RNAs (lncRNAs) appear to have an important role in the development and progression of MS. This study aimed to compare the lncRNAs, MIAT, H۱۹, and NRON in peripheral blood samples of MS cases to a healthy control group. We collected blood samples of MS cases and controls. We focused on the expression of MIAT, H۱۹, and NRON lncRNAs in peripheral blood samples of ۷۶ relapsing-remitting (RR) and ۱۹ secondary progressive (SP) MS cases and compared them with controls using quantitative real-time PCR. Results indicated that MIAT, H۱۹, and NRON expression levels were significantly increased in the RRMS and SPMS subgroups compared to the controls. A positive correlation was found between MS type and the expression levels of MIAT and NRON lncRNAs. Also, the expression level of H۱۹ was correlated with sex in MS patients. Based on the area under curve (AUC) values, NRON had the best performance in the differentiation of MS patients from controls (AUC = ۰.۹۴, P < ۰.۰۰۰۱). A combination of MIAT, H۱۹, and NRON expression levels could be useful in differentiating MS patients with ۹۱.۷۷% sensitivity, ۷۳.۳۵% specificity, and a diagnostic power of ۰.۹۱ (P < ۰.۰۰۰۱). In conclusion, the levels of MIAT, H۱۹, and NRON in peripheral blood could be important biomarkers for MS diagnosis

کلیدواژه ها:

Multiple Sclerosis ، LncRNA ، Biomarkers ، Real-Time PCR