The study of Oct۴ gene expression of mouse blastocysts influenced by embryo splitting

Embryo splitting is widely utilized as a novel technique in reproduction biotechnology. After splitting embryo at the two-, four- or eight-cell stages, each single blastomere can be developed to a separate embryo that is similar to other blastomere genetically. In the present study, the effects of the mouse embryo splitting on the Oct۴, as one of the pluripotent genes, expression were evaluated. After stimulating ovulation in female mice and isolating two-cell embryos, they were grouped as split and non-split, then washed and transferred to M۱۶ medium. When zona pellucida was removed, the blastomeres of the split group were dispersed then, as well as non-split and normal blastomeres, transferred into embryonic fibroblasts to develop. About ۷۲ hours later, normal blastocysts from each group were separately isolated for molecular evaluation. Changes in Oct۴ expression were analyzed by Real-time PCR. The results showed that the pluripotent gene (Oct۴) expression were similar between split and non-split groups. In another words, there was no significant changes in Oct۴ expression, among all the study groups.

کلیدواژه ها:

Embryo Splitting ، Mouse Blastocyst ، Two-cell Embryo ، Oct۴ gene