The effect of niosome nanoparticles encapsulating stevia extract on the expression of TNF- a and IL-۶ genes in type۲ diabetic model induced by intravenous injection of streptozotocin in male rats

Today, diabetes has spread and the drugs used so far have not been completely effective. Therefore, although natural compounds have fewer side effects than chemical compounds, the treatment of diabetes has advanced towards these methods. Therefore, the aim of this research project is to use Stevia Newsome to increase drug efficacy in male diabetic rat models. The purpose of this study was to the effect of niosome nanoparticles encapsulating stevia extract on the expression of TNF- a and IL-۶ genes in type۲ diabetic model induced by intravenous injection of streptozotocin in male rats. In this study, ۴۰ Wistar rats with an average weight of ۲۰۰-۲۵۰ g were used. They were divided into ۴ groups including healthy control, diabetic control (sham), stevia, and niosome stevia. Mice received daily stevia and niosome stevia at a dose of ۵۰۰ mg / kg by gavage for ۳۰ days after diabetes. Forty-eight hours after the last treatment time, the rats in each group were anesthetized and their liver tissue was removed and real-time PCR was performed to examine the TNf- a and IL-۶ genes. Streptozotocin injection significantly increases blood sugar compared to the control group. Injection of stevia with nanoniosome also reduced blood sugar to the control group (p <۰.۰۰۱). Decreased expression of inflammatory factors (TNF- a and IL-۶) (P <۰.۰۱). It can be stated that one of the effects of stevia is the effect on gene expression and the improvement of diabetes, and during this study, the groups that received the drug in the form of niosome further improved this and reduced the expression of the gene. Niosome also produces a better and more effective effect.