Study of preventive effect of alpha-lipoic acid on cisplatin induced hemotoxicity in NMRI mice

Cisplatin is one of the most effective anti-cancer drugs used to treat cancers of ovary, testicles, bladder, head and neck and many other cancers. However, the clinical use of this drug is limited by side effects such as renal, hepatic, neurological, and blood poisoning and its effects on hematopoietic tissues. Experimental studies have shown that oxidative stress caused by the metabolism of this drug is one of the main causes of side effects. Alpha lipoic acid is known as a powerful antioxidant with anti-inflammatory properties. Aim of this study was to evaluate the effect of ALA on improving the blood parameters of cisplatin mice. In this study, ۳۵ adult male mice weighing ۳۰-۴۰ g were divided into ۵ groups of ۷. Control group, cisplatin ۵ mg/kg group, CP+ALA ۵۰ mg/kg group, CP+ALA ۱۰۰ mg/kg group and ALA group ۱۰۰ mg/kg alone received by repeated intraperitoneal injection for ۷ days. At the end of experiment blood sample was taken from heart and parameters of WBC, RBC, HGB, HCT, MCV, MCH, and PLT were analyzed. Our results showed that RBC count, PLT count, level of HGB and HCT significantly were decreased in the CP group compared to control. However, these parameters were increased significantly in the ALA+ CP group compared to the CP group. Although we found a significant increase in WBC and neutrophil count, however, the number of lymphocytes decreased in the CP group compared to the other groups. It is concluded that different doses of ALA has protective effects against CP-induced alteration in parameters of blood cells. Thus, it could be used as a dietary supplementation to reduce CP-induced hemotoxicity.

کلیدواژه ها:

blood parameters ، oxidative stress ، antioxidants ، red blood cells ، white blood cells