Effect of Buxus hyrcana on Rotarod Balance Performance and Oxidative Stress in Experimental Model of Epileptic Male Wistar Rats

Buxus hyrcana has been used in Iranian traditional medicine for anticancer agent. Recently reports were indicating that its extract has also been used to treat epilepsy and other neurodegenerative disorders. The aim of this study was to evaluate the effect of Buxus hyrcana extracts (BHE) on experimental models of seizure-induced motor balance impairment in rats. In this experimental study, male Wistar rats in range of ۲۰۰±۲۰ g were randomly divided to four group of negative control (saline), positive control (PTZ), and BHE treated (۳۰۰, and ۶۰۰, mg/kg, intraperitoneally). All groups except negative control were induced by seizures with sub-threshold dose of pentylenetetrazole (PTZ) (۳۵ mg/kg) every other day for one month and treated with BHE (۳۰۰, and ۶۰۰, mg/kg, intraperitoneally). After full kindling, a standard rotarod test was used to evaluate motor coordination. At the end of experiment rats were sacrificed using deep anesthesia and the hippocampi were isolate for oxidative stress (malondialdehyde (MDA)) evaluation. The data were analyzed by Graphpad Prism software and displayed as mean and standard error from mean. It is also a condition of natural distribution was examined by Shapiro-Wilk test and after ensuring the normal distribution the data were analyzed by one-way ANOVA and subsequent Tukey's post hoc test was performed. Level of less than ۰.۰۵ was considered significant. Motor-skill tasks in rotarod test in PTZ group receiving BHE at a dose of ۳۰۰ and ۶۰۰ mg/kg was greater than PTZ group. In addition, treatment with the BHE protects the hippocampus by lowering the MDA level. Our results showed that BHE could prevent epilepsy and elevate motor coordination in the PTZ-kindled rats by inhibition of oxidative stress damage.

کلیدواژه ها:

Kindling ، Traditional medicine ، Neurodegenerative disorders ، Pentylenetetrazole