Prevalence of the ۱۸ virulence genes in uropathogenic Escherichia coli (UPEC) clinical isolates
محل انتشار: بیست و یکمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 196
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شناسه ملی سند علمی:
MEDISM21_330
تاریخ نمایه سازی: 23 مرداد 1400
چکیده مقاله:
Background and Aim : Escherichia coli is one of the microorganisms most frequently involved in urinary tract infections (UTIs); E. coli strains generally fall into one of four phylogenetic groups A, B۱, B۲, and D and that virulent extra strains belong mainly to group B۲ and, to a lesser extent, to group D. Uropathogenic E.coli (UPEC) show different virulence factors too, such as adhesins, invasins, toxins, siderophores and genes required for capsular biosynthesis. This study aimed to investigate some virulence factors of Uropathogenic Escherichia coli.Methods : One hundred thirty-eight (۱۳۸) uropathogenic E. coli (UPEC) clinical isolates were investigated in this study. Besides, ۳۰ E.coli(samples) as control were collected from the feces of healthy humans. multiplex PCR assays were performed for investigating ۱۸ genes encoding virulence genes finally Chi-square test or Fisher’s exact test was used to compare the occurrence of virulence markers in cases and controls. Results were considered statistically significant at P < ۰.۰۵.Results : From ۱۳۸ (UPEC), ۲۲ (۱۵.۹%), ۶ (۴.۳%), ۰ (۰%), ۲ (۱.۴%), ۲ (۱.۴%), ۱۷ (۱۲.۳%), ۰ (۰%), ۸۳ (۶۰.۱%), ۸۱ (۵۸.۶%), ۴۵ (۳۲.۶%), ۲۰ (۱۴.۴%), ۱۱ (۷.۹%), ۱۸ (۱۳%), ۱ (۰.۷%), ۸ (۵.۷%), ۶ (۴.۳%), ۵۸ (۴۲%) and ۴۵ (۳۲.۶%) isolates presented afa/draBC, bmaE, gafD, nfaE, hlyA, cnf۱, cdtB, fyuA, iutA, kpsMT II, kpsMT III , kpsMT k۱, kpsMT k۵, rfc, ibeA, cvaC, traT and PAI genes.Conclusion : Among ۱۸ VFs, the only statistical difference between abundance the UPEC and commensal isolates was significant for ۳ VFs (fyuA, PAI, and traT). Therefore, confidently it can be said that the frequency of these factors in UPEC isolates is higher than that of commensal isolates.
کلیدواژه ها:
نویسندگان
Mahsa Mirzarazi
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University, Tehran Iran
Seyedeh Elham Rezatofighi
Biology Department of Science Faculty of Shahid Chamran University, Ahvaz, Iran
Mansour Salehi
Genetic Department of School medicine of Esfahan University of Medical science, Esfahan, Iran
Mahdi Pourmahdi
Department of Food Hygiene of Veterinary medicine of Shahid Chamran University, Ahvaz, Iran