Synthesis, characterization, and activity determination of dendrimer-streptokinase conjugated and finding protein crona and Evaloation of cytotoxicity

Background and Aim : Nowadays, dendrimer conjugation with low molecular weight drugs has been considered for improving pharmacokinetics, targeting drugs to particular sites, and helping cellular uptake. Increasing the performance of relatively great therapeutic proteins like streptokinase (SK) using dendrimers is being explored.Methods : In this study, G۲ dendrimer and dendrimer-streptokinase-conjugates were synthesized. After synthesis of dendrimer-SK conjugates, characterization of these conjugates by Fourier-transform infrared radiation (FT-IR), nuclear magnetic resonance (NMR) and high performance liquid chromatography (HPLC) showed that the conjugation reaction was successful.resulting in relatively pure SK-dendrimer conjugates and checked the cytotoxicity effect.Results : Here, we show that dendrimer-streptokinase conjugates formed crona protein in whool blood cells.hese results emphasize the need of investigating the formation and biological importance of the protein corona in the blood as the nanoparticles are intended for or released into. Moreover, potency of dendrimer-SK conjugates was greater than streptokinase. It seems that high enzymatic activity of dendrimer-SK conjugates can be a favorable way for changing bioactive macromolecules with dendrimer. Furthermore, injection of dendrimer-streptokinase conjugatesmay be promising for treatment of many conditions.Conclusion : Dendrimers were useful for effective attachment to various proteins. It seems that high enzymatic activity of dendrimer-SK conjugates could be a favorable way for changing bioactive macromolecules with dendrimer. Furthermore, injection of dendrimer-streptokinase conjugatesmay be promising for treatment of many conditions

کلیدواژه ها:

Dendrimer ، streptokinase. conjugation ، potency ، characteristic ، Protein crona