The effect of Nano-Curcumin on biofilm regulatory genes of Pseudomonas aeruginosa

Background and Aim : Pseudomonas aeruginosa is a pathogen that causes nosocomial infections, especially in immunodeficient patients. The biofilm has an important role in the virulence of P. aeruginosa and is under the regulation of the Quorum sensing of bacteria. Curcumin, an active phenolic extract of turmeric has shown an inhibitory effect on the biofilm formation of some pathogenic bacteria. Thus, this study aims to evaluate the effect of Nano-Curcumin on the expression of biofilm regulatory genes of P. aeruginosa.Methods : The biofilm formation of P. aeruginosa ATCC ۱۰۱۴۵ was assessed in the presence of ۱۵, ۲۰, and ۲۵ μg/ml concentrations of Nano-Curcumin using the microplate titer method. The effect of Nano-Curcumin on the expression of evaluated genes was determined by relative Real-time PCR.Results : In the absence of Nano-Curcumin, P. aeruginosa strain ATCC ۱۰۱۴۵ strongly produced biofilm (۳+) and in the presence of ۱۵ and ۲۰ μg/ml, biofilm formation was reduced to moderate (۲+) and weak biofilm producer (۱+), respectively. Nano-Curcumin at a concentration of ۲۵μg/ml inhibited biofilm formation in P. aeruginosa. The expression of regulatory genes was not affected by biofilm inhibitory concentrations of Nano-Curcumin.Conclusion : The antibiofilm mechanism of Curcumin is not related to the exchange of genes involved in biofilm formation of P. aeruginosa and probably it inhibits the biofilm intact structure.

کلیدواژه ها:

Pseudomonas aeruginosa ، Biofilm formation ، Nano-Curcumin