Investigation on Urinary Tract Infections Following C-section and Assessment of CTX-M and TEM Genes Prevalence in Escherichia coli and Klebsiella Causing the Infections in Lamerd
محل انتشار: بیست و یکمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 224
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شناسه ملی سند علمی:
MEDISM21_154
تاریخ نمایه سازی: 23 مرداد 1400
چکیده مقاله:
Background and Aim : : Antibiotics are usually prescribed by surgeons before and after surgery to prevent postoperative infections. Prophylaxes used for cesarean section in Lamrerd are cefazolin and cephalexin. According to studies carried out, urinary tract infections can also occur in spite of the above prescribed antibiotics. So, this research was carried out to determine those strains resistant to the mentioned antibiotics and also to identify genes causing resistance in antibiotic-resistant strains.Methods : : ۱۴۰ cases of mothers who had cesarean section (C-section) were considered in this study. The research began in April and lasted till July ۲۰۱۴ for case selection and sampling. It was emphasized to those women delivered via C-section to refer to laboratory ۱۵ days after delivery. UA-UC tests were done on them. Phenotypic confirmatory test was performed for cefazolin and cephalexin antibiotic-resistant strains in the presence of ceftazidime, ceftazidime/clavulanic acid and cefotaxime, cefotaxime/clavulanic acid discs. The presence of CTX-M and TEM genes in the resistant strains were examined finally by performing PCR test.Results : : Of ۱۴۰ studied C-sections, ۱۰ cases of urinary tract infections including ۳ cases of E. coli (۲.۱%), ۱ Klebsiella (۰.۷), ۲ cases of Proteus (۱.۴%) and ۴ cases of Staphylococcus (۲.۸%) were observed. According to phenotypic confirmatory test, Escherichia coli and Klebsiella strains were isolated as strains containing ESBL for next studies. Finally, according to PCR test for Escherichia coli and Klebsiella isolates, all of them contained CTX-M and TEM genes.Conclusion : : It is necessary that microbial culture and antibiogram tests to be done before administering any antibiotics, in order to prevent the spread of strains containing ESBL with proper prescription of antibiotics.
کلیدواژه ها:
نویسندگان
Hushang Jamali
Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
Davood Irani
Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran