Phylogenetic group distributions in Uropathogenic Escherichia coli Strains Isolated from Patients with Urinary Tract Infection; Southwest Iran

Background and Aim : Urinary tract infections (UTI) are the most commonly encountered infections in clinics and outpatient settings and are mainly caused by Uropathogenic Escherichia coli (UPEC). Phylogenetic analyses have shown that Escherichia coli isolates fall into four main phylogenetic groups A, B۱, B۲, and D. Biofilm formation is the pathogenic process that allows E. coli to be maintained in the urinary system and prevents the loss of bacteria. The aim of this study was to identify the phylogenetic groups of UPEC strains using Clermont’s triplexPCR method and to assess Biofilm formation of E. coli isolates from UTI cases in Yasuj, IranMethods : A total of ۱۳۰ E.coli isolates were collected from patients with UTI during July to November ۲۰۱۷. Biochemical and standard microbiological techniques were used to identify E.coli and the presence of phylogenetic groups’ genes was investigated by multiplex polymerase chain reaction (PCR). Antimicrobial resistance pattern was performed by Kirby-Bauer method and Biofilm formation was detected by Congo Red Agar (CRA) phenotypic assayResults : The phylogenetic analysis of the UPEC isolates revealed that most of the isolates belonged to groups B۲ and D. The isolates from UTI cases were distributed within phylogroups B۲ (۷۴.۶۱%), D (۱۵.۳۸%), B۱ (۶.۱۵%), and A (۳.۸۴%). There was a significant association between the formation biofilm and antimicrobial agents tested including Ampicilin (p-value=۰.۰۲۰) and Co-Trimoxazole (p-value=۰.۰۳۸).Conclusion : The phylogroups of E. coli isolates from UTI cases showed that groups B۲ and D are prevalent in in E. coli strains isolated from patients with UTI in Yasuj. Therefore, phylogenetic group B۲ plays a more effective role than other phylogenetic groups in urinary tract infection caused by Escherichia coli bacteria in this area. Detection Phylogenetic group distributions, biofilm in E. coli and its resistance to commonly prescribed antibiotics in the clinical practice is essential in improving the efficacy of empirical treatment.

کلیدواژه ها:

Antibiotic Resistance ، Biofilm ، Escherichia coli ، phylogenetic group ، Urinary Tract Infection ، Uropathogenic