Evaluation of antibiotic profile and biofilmogenicity ability in Klebsiella pneumoniae isolates with broad-spectrum beta-lactamase (ESBL) resistance

Background and Aim : Klebsiella pneumoniae is a gram-negative bacterium that can cause a variety of infections, including nosocomial infections. Various infections caused by Klebsiella pneumoniae with annual carbapenemic resistance cause high mortality and treatment costs in various countries around the world. The aim of the present study, which was conducted as part of the dissertation project, is to investigate the antibiotic profile and the biofilmability capacity of Klebsiella pneumoniae isolates with ESBL resistance in ۱۳۹۸Methods : ۷۳ isolated Klebsiella pneumoniae were collected from patients in hospitals and laboratories in Tehran province and after confirmation of the samples with standard biochemical experiments, ESBL isolates were screened by combined phenotypic combination method. Then, the antibiotic profile of the obtained samples, along with the ability to form biofilm by microplite method in laboratory conditions were examined.Results : Of the ۷۳ samples, ۴۴ (۶۰%) had broad-spectrum beta-lactamase enzymes. The highest antibiotic resistance was observed with ampicillin, chloramphenicol and ciprofloxacin antibiotics, and the lowest resistance was related to gentamicin, amikacin and cotrimoxazole. Most samples were able to form biofilm.Conclusion : Comparing the present study with previous studies, we saw an increase in antibiotic resistance in the studied isolates, and unfortunately the prevalence of isolates with high beta-lactamase resistance was high. It is recommended that the isolates be tested for ESBL resistance genes.

کلیدواژه ها:

Klebsiella pneumoniae ، ESBL ، Biofilm ، Antibiogram