Determine Of The Prevalence of human papillomavirus (HPV-۱۶) infection in lesions of lichen planus in comparison with oral lichenoid using PCR

Background and Aim : human papiluma virus (HPV) cause oral, cervical, and any other keratinocyte cancers and are of ۲ groups based on the risk of carcinogenesis; high-risk and low-risk HPVs. Oral lichen planus (OLP) is a common chronic autoimmune disorder, which causes damages to the keratinocytes of the oral mucosa. Although the etiology of OLP is still unknown, it is generally accepted to be a T-cell-mediated inflammatory disease. The reaction of these specific CD۸ T cells is similar to what occurs during a viral infection, in which a virus can act as a cytoplasmic antigen or induce the expression of host cell proteins. Oral lichenoid lesions (OLLs) are a group of lesions with different etiologies but common clinical and histopathological features. Considering the previous studies, it was reported that the premalignant potential of OLLs could be more than that of OLPs. Since the HPV virus in OLLs has not ever been studied this research aimed at investigating the presence of HPV-۱۶ (human papillomavirus) in Oral lichenoid in order to evaluate a possible association between premalignant potential of HPV in lichenoid lesions in comparison to Oral lichen planus.Methods : fifty formalin fixed paraffin wax embedded tissue blocks ۲۵ paraffin block of OLPs as well as ۲۵ formalin fixed paraffin wax embedded tissue blocks of of OLLs, which were previously diagnosed, were gathered from patients who had referred to the Department of Pathology, Faculty of Dentistry of Islamic Azad university, Isfahan (Khorasgan) Branch during ۲۰۱۳-۲۰۱۸. DNA extraction from formalin fixed paraffin wax embedded tissue blocks was performed using GEN ALL extraction kit. Confirmation of the extracted DNAs was conducted using β-globin gene. The samples were tested for the presence of HPV-۱۶ genome by PCR .Results : In the two experimental groups, the presence frequency of HPV-۱۶ was detected respectively at ۰ and ۶.۷ % of OLLs and OLPs. Fisher’s test did not show this difference statistically significant (P value> ۰.۰۵).Conclusion : Based on the findings of this study it is conceivable that HPV-۱۶ plays a role in the creation of OLLs. According to the obtained results, performing further studies in this field is recommended.

کلیدواژه ها:

PCR ، Oral lichenoid ، HPV-۱۶