Arbutin attenuates nephrotoxicity induced by gentamicin

Objective: In this study, the impact of arbutin was examined in a gentamicin (GM)-induced nephrotoxicity model. Materials and Methods: Forty adult male Wistar rats were randomly assigned to five groups including control group; GM group, and three groups of GM+arbutin (۲۵, ۵۰ and ۷۵ mg/kg). One day after the last injection of GM, creatinine, urea, carbonyl, thiobarbituric acid-reacting substance (TBARs), ferric reducing antioxidant power (FRAP) and ۸-hydroxyguanosine levels were assessed in serum samples. Left and right kidneys were used for biochemical assays and histological evaluation, respectively. Results: Our data showed that the FRAP level (p<۰.۰۵), urea (p<۰.۰۰۱), creatinine (p<۰.۰۰۱), and ۸-hydroxyguanosine (p<۰.۰۰۱) levels of serum samples, were increased in GM-treated rats compared to the controls. The serum levels of TBARS (p<۰.۰۰۱) and carbonyl increased in serum and renal tissue (p<۰.۰۰۱) of GM-treated animals. Conversely, arbutin attenuated serum creatinine, urea and ۸-hydroxyguanosine, and TBARS (p<۰.۰۰۱). Administration of arbutin significantly decreased carbonyl levels in serum and renal tissue samples (p<۰.۰۰۱). Furthermore, the levels of FRAP increased in the serum (p<۰.۰۱) and renal tissue samples (p<۰.۰۰۱) of arbutin-treated animals. Histological staining showed that arbutin significantly inhibits kidney damages. Conclusion: Our data suggest that arbutin attenuates GM-induced nephrotoxicity through its free radicals-scavenging activity.

کلیدواژه ها:

Gentamicin ، Nephrotoxicity ، Arbutin ، Antioxidant ، Histopathology