Evaluation of V۶۱۷F JAK ۲ gene mutation by high resolution Melting method in patient witherythrocytosis

Although several techniques have been developed for the detection of JAK۲ V۶۱۷F mutation, these techniques have their disadvantages. High-resolution melting (HRM) analysis is a new, post-PCR analysis. Simple and fast, this method is based on PCR melting curve techniques. This study examined the JAK۲ V۶۱۷F mutation by the high-resolution melting method in ۲۰ patients with erythrocytosis, and the results were compared with those obtained from the direct sequencing method. The results showed ۱۰۰% sensitivity and ۱۰۰% positive predictive value for this methodology in the patient sample set tested.

کلیدواژه ها:

Myeloproliferative ، JAK۲ ، point mutation ، High-Resolution Melting