Evaluation and comparison of the efficiency of different molecular markers in estimating genetic distance of different Persian oak (Quercus brantii Lindi.) populations

Background and Aim: Estimation of genetic diversity and evaluation of plant natural resources is the most important step in the collection and management of germplasm. DNA-based genetic markers, in addition, not only to identifying genetic distances between and within natural plant populations, but also determine patterns of genetic structure alteration and factors that influence it. However, comparing different markers and providing the most efficient markers is very important. In order to investigate genetic variation among Persian oak (Quercus brantii Lindi.) populations of Lorestan province, ۲۰ genotypes were collected from different geographical and climatic regions. After DNA extraction, polymerase chain reactions (PCR) were used for amplification using three markers of ISJ, ISSR and SCoT. Genotyping was performed polymorphic bands obtained from all three markers separately, and the marker data were combined and final grouping determined. The obtained groupings were compared and based on the obtained components. PCR results of the primers showed ۹۱ polymorphic bands with an average of ۷۱% per locus. The ISSR marker with ۴۴ polymorphic bands was the highest. Genotypes formed by ISJ, ISSR and SCoT markers in groups ۵, ۶ and ۵, respectively, and grouping from three marker combinations showed ۵ groups. The results showed that the obtained clustering for different markers and the combination of ۳ markers were consistent with the existing climatic grouping so that the genotypes belonging to a similar climatic region were grouped in similar clusters. The most similarity between the groupings was between ISJ and ISSR markers with ۸۹%. Overall, the results indicated the usefulness of markers used to estimate genetic distances between different oak communities. It seems that using several different marker systems to cover a wider and more diverse genome level could be useful in more accurate estimation of genetic diversity and distances.Methods: Genetic variation among Persian oak (Quercus brantii Lindi.) populations of Lorestan province were evaluated, ۲۰ genotypes were collected from different geographical and climatic regions. After DNA extraction, polymerase chain reactions (PCR) were used for amplification using three markers of ISJ, ISSR and SCoT.Results: Polymerase chain reactions were used for amplification using three markers of ISJ, ISSR and SCoT. Genotyping was performed polymorphic bands obtained from all three markers separately, and the marker data were combined and final grouping determined. The obtained groupings were compared and based on the obtained components. PCR results of the primers showed ۹۱ polymorphic bands with an average of ۷۱% per locus. The ISSR marker with ۴۴ polymorphic bands was the highest. Genotypes formed by ISJ, ISSR and SCoT markers in groups ۵, ۶ and ۵, respectively, and grouping from three marker combinations showed ۵ groups. The results showed that the obtained clustering for different markers and the combination of ۳ markers were consistent with the existing climatic grouping so that the genotypes belonging to a similar climatic region were grouped in similar clusters. The most similarity between the groupings was between ISJ and ISSR markers with ۸۹%.Conclusion: Results indicated that usefulness of markers used to estimate genetic distances between different oak communities. It seems that using several different marker systems to cover a wider and more diverse genome level could be useful in more accurate estimation of genetic diversity and distances.

کلیدواژه ها:

Persian oak ، Genetic diversity ، Zagros ، Molecular markers