The comparison of expression circ Amotl۱/hsa_circ_ ۰۰۰۴۲۱۴ as a biomarker in breast cancer tumor tissues and bloods of patients with breast cancer and non tumor tissues and normal bloods

Background and Aim: Breast cancer is one of the most common malignancy whit leading cause of death from cancer in women in worldwide.thus,methods for the early diagnosis,accurate prediction and appropriate are very important. Finding a Biomarker have an important role in the prognosis and early diagnosis of breast cancer. Recently, the roles of Circular RNAs as a biomarker in cancer have attracted much attention. Circular RNA as a type of non-coding RNAs that form aloop with jointed ۳' heads and ۵' tails. Circ RNA has been indicate to play a crucial role in growth, differentiation, metabolism and metastasis with sponge miRNA and promotes cell proliferation, migration and invasion and have role in tumor growth in breast cancer. In the present study rate of expression circ Amotl۱/hsa_circ_ ۰۰۰۴۲۱۴ as a biomarker is compared in breast cancer tumor tissues and bloods of these patients with non tumor tissues and normal bloods.Methods: From ۳۰ tumor tissue samples and bloods of these patients and ۳۰ non tumor tissue samples and bloods of them RNA extraction was carried out with TRIZOL reagent and precipitated with phenol chloroform and ethanol. CDNA was synthesis with the reverse transcription kit. The primer for Circ_۰۰۰۴۲۱۴ designed. Subsequently RT PCR was accomplished with SYBR Green Master Mix in accordance with the manufactures protocol. The data were analyzed using the ۲−∆∆ct method and normalized to β-actin levels.Results: The RT PCR analysis revealed that circ Amotl۱/hsa_circ_ ۰۰۰۴۲۱۴ were up regulated in breast cancer tissues compared with that in normal tissue and also were up regulated this circ in bloods of these patients compared with normal blood. Conclusion: We highlighted that hsa_circ_ ۰۰۰۴۲۱۴ as a novel prognostic marker for breast cancer. Thus, this circular RNA might be a potential therapeutic target for breast cancer prognosis and early diagnosis and treatment.

کلیدواژه ها:

breast Cancer ، circular RNA ، Biomarker ، hsa_circ_۰۰۰۴۲۱۴