SNPs with a probable role in over-expression of FAIM۲ and prediction of related molecular networks

Background and Aim: Fas apoptotic inhibitory molecule ۲ (FAIM۲) is a member of the Life Guard Family (LFG) contributed to the apoptotic pathways. FAIM۲ as an anti-apoptotic molecule inhibits the creation of FAS apoptotic cascade; therefore, a considerable increase in this transcript levels brings about a repression of apoptosis associated with FAS, which is restated in some cancers such as lung cancer. MiRNAs are a group of non-coding RNAs that would decrease the level of FAIM۲ expression through post-transcriptional regulation. Alteration in a target site of the above-mentioned gene would affect the connection between miRNA and the oncogene transcript. In the current study, we aim to investigate SNP within target sites and analyze the possibility of disturbance in the aforementioned connection, as well as interaction provided between FAIM۲ and other molecules.Methods: To find miRNAs possessing connection sites in FAIM۲, databases such as StarBase, miRTarBase, and miRBase were used. Then, SNPs detected in target sites using TargetScan were analyzed by Ensemble. GeneMANIA server was used so that network analysis and prediction of the ontology of target genes.Results: Bioinformatic analysis indicate there are some miRNAs possessing target sites on FAIM۲, some SNPs distinguished in such sites, and also some related genes to FAIM۲ were defined.Conclusion: In this study, we found SNPs in some target sites of FAIM۲ with the possibility of changing the expression level of that. Alteration in the level of FAIM۲-mRNA represses the FAS signaling pathway -one of the apoptotic pathways- which is proved in some cancers. Not to mention, the expression level of FAIM۲ will not be controlled by miRNA if any disturbance is caused among the grabbing of this oncogene by miRNA. SNPs are possible reasons. In the current study, we came to the conclusion that there are ۱۶ SNPs associated with target sites with a probable role in the soaring level of FAIM۲. FAIM۲, meanwhile, displays physical interaction or co-expression with ۲۰ molecules.

کلیدواژه ها:

FAIM۲ ، SNP ، Cancer ، Post-transcriptional regulation