Study of miR-۳۴۲-۳p expression profile in human breast cancer

Background and Aim: MicroRNAs (miRNAs) are small non-coding RNAs that contribute to modulating signaling pathways after radiation exposure and have emerged as a potential therapeutic target or biomarker in the radiation response of cancer. miR-۳۴۲-۳p is lower in pre-menopausal inflammatory breast carcinoma patients, associated with ER levels. This study was aimed to compare miR-۳۴۲-۳p expression profile in human breast cancer using RT-qPCR.Methods: Twenty samples from patients with breast cancer and twenty samples from non-tumor were collected by a pathologist specialist. After RNA extraction from tumoral and normal tissue samples, cDNA was synthesized according to protocol and RT-qPCR carried out using SYBR®Premix Ex TaqTM II kit. miR-۳۴۲-۳p expression profile was analyzed by ΔΔCT method.Results: Our results revealed that the mean of miR-۳۴۲-۳p expression profile in human breast cancer is down-regulated in comparison with control group.Conclusion: Overall, the results of the present study revealed distinct differences in the expression profile of miR-۳۴۲-۳p to control group (down-regulation of miR-۳۴۲-۳p), and this miRNA can be used as biomarkers for cancer therapy

کلیدواژه ها:

miR-۳۴۲-۳p ، Breast cancer ، RT-qPCR.