Investigation of Eryngium billardieri extract on PPARa gene expression on colon cancer

Background and Aim: Colon cancer, which is the third most common cancer in the world, begins in the large intestine. Since polyps are the first step of colorectal cancer development, their removal is considered as preventive treatment .in addition, it has been demonstrated that expression of PPAR gene as an inhibitor of cell proliferation, suppresses the development of colorectal tumors Although various chemotherapy regimens have been suggested recently, the mortality rate of this cancer remains high. Regarding the devastating adverse effects of chemotherapy drugs and the low compliance of patients receiving chemotherapies, finding new treatment choices are still of a concern. On the other hand, recent studies have reported the medicinal herbs and Phyto-compounds as beneficial complementary treatments in cancer. Eryngium billardieri, a member of Umbelliferae family, is widely used as a medicinal plant for the treatment of various diseases. Root extracts and aerial parts show anti-inflammatory and anti-hyperglycemic effects. Moreover, this plant induces apoptosis besides having antitumor and antibacterial activities. Regarding the possible effect of Eryngium billardieri on tumor cells and the role of PPARa in inhibiting the cell proliferation, the aim of this study was to investigate the effect of ethanolic extract of Eryngium billardieri flowers on PPARa expression in colorectal cancer cell line.Methods: Thirty-five colon tumor and paired adjacent non-tumor tissue samples were collected from the Emam-Reza Hospital, Tabriz, Iran. We measured the expression of PPARa by qRT-PCR. Then, we investigated the effect of Eryngium billardieri extract on the expression of PPARa in colon cancer cell line (HT۲۹) at RNA levels Results: Our results showed a significantly higher expression of PPARa in tumor tissues as compared to non-tumor tissues (P=۰.۰۲۸). Eryngium billardieri extract treatment substantially attenuated PPARa expression in HT۲۹ cells.Conclusion: PPARa was overexpressed in colon cancer tissues as compared to the non-tumor tissues.In addition, Eryngium billardieri extract treatment attenuated PPARa expression in colon cancer cells.

کلیدواژه ها:

eryngium billardieri ، colon cancer ، ht۲۹ ، cell proliferation