Association of Vitamin E Level in Follicular Fluid with Morphology of Oocyte and Quality of Embryo in IVF Patients, Alzahra Hospital Rasht

Background and Objective: As a result of globally increasing trend in infertility, taking advantage of reproductive technology to deal with this issue is recommended. Taking the well-founded claim about Vitamin E as a powerful antioxidant with a protective role in the body, this study was instigated to find the relationship between levels of vitamin E in Follicular fluid and morphology of oocyte along with embryo quality in IVF patients. Materials and Methods: This cross-sectional analytical study was carried out on oocytes and embryos obtained from ۵۰ women with IVF admitted to Al Zahra Hospital of Rasht city. Patients underwent a similar ovulation stimulation protocol and then ten thousand units of hCG injected. After ۳۶ hours, they underwent Follicle suction and mature oocytes were isolated for fertilization. Vitamin E level was measured and consequently the morphology of oocytes and the quality of embryo were evaluated by means of inverted optical microscopy. Results: Of ۵۰ treated IVF women, ۵۸۳ oocytes and ۲۷۵ embryos were examined. No significant relationship was observed between age, BMI, duration or cause of infertility and Vitamin E (P>۰/۰۵). At ۰.۵-۱ mg/dl level of vitamin E, there were higher MII oocytes in comparison with other levels (p= ۰.۰۱۴). Despite the mean number of ۲PN embryos at this level of vitamin E was more than other levels, this difference was not statistically significant (P=۰.۸۷۲). Conclusion: The results prove the evidence that at ۰.۵-۱mg/dl level of Vitamin E, oocyte morphology and embryo quality were significantly improved.  

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Keywords: Infertility ، Follicular Fluid ، IVF ، Vitamin E ، Oocytes ، Embryo ، Antioxidants