A Study on Cephalosporin Acylase Enzymes in Some Gram-Negative Bacteria Isolated from Clinical Samples for Producing Cephalosporin Semi-Synthetic Antibiotics in Pharmaceutical Industries
سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 191
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شناسه ملی سند علمی:
JR_ZUMS-21-89_005
تاریخ نمایه سازی: 11 اردیبهشت 1400
چکیده مقاله:
Background and Objective: Cephalosporin acylases (CA) are highly specialized peptidases that are able to break down the amide bond between the lactam nucleus and lateral chain without destruction of beta-lactam ring. This enzyme transmutes the cephalosporin C to ۷-ACA. The ۷-ACA is an important intermediate in synthesizing the semi-synthetic cephalosporin in pharmaceutical industries. The purpose of this study was to screen and optimize the species having maximum amount of production of cephalosporin acylase enzyme isolated from clinical samples among some gram- negative bacteria producing cephalosporin acylase. Materials and Methods: Out of a total of ۶۰ species gathered from several hospitals in different places of Tehran city, the clinical species such as Pseudomonas, Acintobacter and Escherichia coli were isolated. At first, the resistant species were differentiated by antibiogram method. Then, the activity of enzyme was measured by spectrophotometer. After identification and isolation of the enzyme producing species, the effect of factors such as time, temperature, pH and the amount of aeration of cultivation environment on the amount of enzyme production was investigated. Results: In this study, among the three groups of bacteria mentioned above, a species of Pseudomonas aeroginosa showed a significant cephalosporin acylases activity. Optimum activity for this species regarding the mentioned factors was estimated as follows: pH ۸, incubation time ۴۸ h, temperature ۳۷ C and the component of aeration was ۱۵۰ rpm. Conclusion: The findings point out that Pseudomonas species has a significant cephalosporin acylases activity compared with other species examined in this study. Therefore, this species may have implication for industrial production of the above mentioned enzyme and also can be used in related researches.
کلیدواژه ها:
Keywords: Cephalosporin acylase ، Optimization ، Cephalosporin ، Cephalosporin -۷ - Aminocephalosporanic acid ، Pathogenic gram-negative bacteria
نویسندگان
فرزانه حسینی
Tehran Azad University, North Branch, Tehran, Iran
عباس اخوان سپهی
Tehran Azad University, North Branch, Tehran, Iran
مائده ورقائی
Tehran Azad University, North Branch, Tehran, Iran