Preparation and Characterization of New Porous Graphene Oxide-based Scaffolds for Bone Tissue Engineering

Scaffolds are a key requirement in bone tissue engineering, because can mimic the structure and function of the extracellular matrix (ECM) and support cell adhesion, proliferation and differentiation. In recent years, significant development has been achieved in the construction of artificial bone with ceramics, polymers and metals. Nano-hydroxyapatite (nHA) is an attractive bioceramic for hard tissue repair and regeneration due to its physicochemical similarities to natural apatite.1 nHA with natural or biopolymer-basd composites containing chitin, collagen, gelatin, fibrin and with synthetic polymers such as polylactic acid, polycaprolactone, poly (lactic-co-glycolic) acid, polyurethane and polyhydroxybutyrate improve cellular attachment, viability, proliferation, and activity as well as mechanical properties.3 Graphene and its derivatives such as graphene oxide (GO) have been considered as an attractive substrate for stem cell culture, as culture on these materials can enhance proliferation and lineage specification of the stem cell.3These unique properties make GO-based materials promising candidates as a scaffold for bone regeneration.In this work, the porous GO-nHA/collagen/PU-polymer composite scaffolds containing different amount of GO were prepared. Interactions between in the individual components in the formation of the composits were confirmed by FTIR and XRD. The porous morphology of scaffolds was confirmed by SEM images. Osteoconductivity and biocompatibility of scaffolds were confirmed by in vitro MTT assay.

کلیدواژه ها:

Scaffold ، Hydroxyapatite ، Graphene oxide ، Bone tissue engineering.