Characterization and frequency of antibiotic resistance related to membrane porin and efflux pump genes among Acinetobacter baumannii strains obtained from burn patients in Tehran, Iran

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 251

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شناسه ملی سند علمی:

AVAN01_018

تاریخ نمایه سازی: 5 بهمن 1399

چکیده مقاله:

Background: To explore the characterization and frequency of antibiotic resistance related to membrane porin and efflux pump genes among Acinetobacter baumannii (A. baumannii) strains obtained from burn patients in Tehran, Iran.Methods: In this cross-sectional descriptivestudy, 100 strains of A. baumannii isolated from burn patients visiting teaching hospitals ofTehran were collected from January 2016 to November 2017. After A. baumannii strains wereconfirmed, antimicrobial susceptibility testing was done via Kirby-Bauer disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines. PCR amplificationwas performed for detection of 毬-lactamase adeR, OprD, adeS genes among A. baumannii strains.Results: All isolates (100%) were resistant to ceftazidime, cefotaxime, cefepime,ciprofloxacin, and piperacillin, and most isolates indicated high resistance (95%-97 ) WR meropenem, imipenem, gentamicin, ceftriaxone, trimethoprim-sulfamethoxazole, piperacillintazobactam, amikacin, and tetracycline. The most effective antibiotic against A. baumanniiisolates was colistin (97% sensitivity), followed by tigecycline. The frequency of OprD, adeS,and adeR genes were 98%, 91%, and 77%, respectively.Conclusions: This study shows for the majority of A. baumannii isolates are highly resistant to the antibiotics most commonly used in burn patients. Also, high distribution of OprD and adeRS genes may be responsible for the observed resistances among A. baumannii isolates that demonstrate the possible role ofboth efflux pumps in simultaneous of carbapenemase production during antibiotic resistance

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نویسندگان

Sousan Akrami

Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Majid Noori

Golestan Hospital, Army University of Medical Sciences, Tehran, Iran , Department of Microbiology, school of Medicine

Behzad Mohsenzadeh

Golestan Hospital, Army University of Medical Sciences, Tehran, Iran , Department of Microbiology, school of Medicine

Aghil Bahramian

Shahid Beheshti University of Medical sciences, Tehran, Iran , Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Fatemeh Shahi

Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Habibollah Mirzaei

Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, IR, Iran