The first report and molecular characterization of Dactylogyrus minutus Kulwiec, 1927 (Monogenea: Dactylogyridae) from cultivated common carp (Cyprinus carpio) in Iran

Common carp (Cyprinus carpio) is one of the most popular farmed cyprinids in Iran. In this study 112 common carp (Cyprinus carpio) were collected from 10 fish farms in Guilan province. In total 5427 Dactylogyrus specimens were isolated and identified using morphometric measurernents of hard parts, the morphology of haptoral parts and the shape of the male copulatory organ. Four species D.extensus, D.anchoratus, D.vastator and D.achmerowi were previously reported but This is the first report of Dactylogyrus minutus from Iran as a new locality report. The prevalence of D. minutus in fish specimens was %11.6. The highest infection of D. minutus was recorded in winter. For molecular investigation the genomic DNA was extracted from one parasite specimen and the 28S rDNA and 18S rDNA regions of specimens were amplified by related primers in PCR. Sequences were deposited in GenBank with accession numbers MF926269.1 and MG821489.1 for the 28S rDNA and 18S rDNA regions respectively. This is the first record of 18S and 28S genes of D. minutus in NCBi GenBank. These findings provide a foundation for future research in to the genetic make-up of dactylogirid family and also for parasite identificatin. The tree topologies derived from the phylogenetic analysis depicted that D. minutus and D. extensus are genetically closely related

کلیدواژه ها:

common carp ، molecular ، Guilan ، Dactylogyrus minutus