Bioinformatics study of single nucleotide polymorphism rs2279744 and hsa-miR-10a-3p related to it in Acute Lymphoblastic Leukemia

Introduction & Objectives: Leukemia is the fifth most common cancer in the world. Acute lymphoblastic leukemia is the most common type of leukemia in children. MDM2 is one of the important genes in this cancer. MDM2 plays an important role in P53 degradation. Increaseing levels of MDM2 protein, promotes tumor formation. Rs2279744 is located on the MDM2 gene and has T alleles (dominant allele) and G allele (recessive allele or SNP). The aim of this study was to evaluate the association of rs2279744 polymorphism and related hsa-miR-10a-3p, due to the role of MDM2 gene in leukemia cancer pathway.Materials & Methods: In the first step, TAEGETSCAN7.2 database and hsa-miR-10a-3p were used to identify microRNAs associated with MDM2 gene. This microRNA binds the region of rs2279744 single nucleotide polymorphism. By confirming the role of has-miR-22-5p in leukemia via the phenomiR database, it was found that this microRNA is down-regulated in leukemia. The hsa-miR-10a-3p target genes were identified from the miRWALK2.0 database and the related signal pathways were analyzed by using the DAVID and KEGG databases.Results: According to the data obtained from paperes, the risk of SNP allele in cancer in the population was considered as a research hypothesis for this polymorphism. According to the pathway obtained from the KEGG database, being oncogeneic was confirmed for the MDM2. Due to the decreased expression of hsa-miR-10a-3p, less inhibited MDM2 will result in increased expression.Conclusion: In fact, in the presence of SNP allele, gene is overexpressed and overly degrades p53 (tumor suppressor protein). It can be predicted that the presence of the SNP allele in rs2279744 increases the possibilityof being prone to cancer. It is expected that by designing primer at rs2279744, the SNP allele will be in leukemia patients by in-vitro testing. On the other hand, this is a possibility that the presence of the SNP allele decrease the risk of cancer.

کلیدواژه ها:

leukemia ، MDM2 ، hsa-miR-10a-3p ، rs2279744