Bioinformatics study of single nucleotide polymorphism rs3733890 and hsa-miR-185-5p related to it in Acute Lymphoblastic Leukemia

Introduction & Objectives: Acute lymphoblastic leukemia accounts for 80% of childhood leukemia. BHMT is one of the important genes in this cancer. One of its important functions is the production of methionine. Rs3733890 is located on the BHMT gene and contains the G allele (dominant allele) and the A allele (recessive allele or SNP). The aim of this study was to evaluate the role of BHMT gene and its association with has-miR-185-5p in the pathway of acute lymphoblastic leukemia.Materials & Methods: At first, the TARGETSCAN database was used to identify BHMT-related microRNAs. After investigating the binding site of related microRNAs, hsa-miR-185-5p was selected. This microRNA binds to the region that rs3733890 is located. The target genes hsa-miR-185-5p were obtained from the miRWALK2.0 database. The associated signal pathways were identified by using the DAVID and KEGG databases.Results: Based on the information obtained from the articles, the risk of SNP allele for this polymorphism was considered as a research hypothesis. Although according to the articles, hsa-miR-185-5p is a tumor suppressor that has been downregulated in cancer. Due to downregulated expression, microRNA less binds to the gene and has less inhibitory effect. In this case, gene expression is increased.Conclusion : When the SNP allele is present, BHMT have been overexpression due to less inhibition by hsa-mir-185-5p and leads to cancer pathway. As a result, this gene is an oncogene in the cancerous pathway. Increased gene expression leads to increased methionine production and feeding of tumor cells to methionine and promote cancer. Based on the research hypothesis, it is expected that by designing primer at rs3733890, the SNP allele will be in leukemia patients with in-vitro test. It is predicted that the presence of the SNP allele in the BHMT gene may increase the risk of cancer and can be used as a prognostic factor for acute lymphoblastic leukemia.

کلیدواژه ها:

leukemia ، BHMT ، hsa-mir-185-5p ، rs3733890