Designing a potent epitope based vaccine against brucellosis using bioinformatics approaches

Background: Brucellosis is a one of the most important zoonosis that caused by Brucella bacteria. Brucella abortus is a common Brucella species associated with Human‑to‑human transmission. Therefore, having a potent vaccine against this bacterium in human will be promising. Therefore, we designed a peptide based vaccine according to outer membrane lipoproteins of Omp 10 and Omp 16 proteins using immunoinformatics approaches. This vaccine provides physicochemical properties such as stability in room temperature, potential of antigenicity, non-allergic properties and no requirement with eukaryotic host system. Also, this vaccine can interact with TLR-4.Results: The designed vaccine has two epitopes which promotes cellular immunity and INF-γ responses. In order to increase immunogenicity of designed vaccine, HBHA as a toll like receptor 4 (TLR-4) agonist incorporated in vaccine structure. Structure of the vaccine was predicted using I-TASSER and refined in GalaxyRefine. In the next step, this refined structure was validated using Rampage and ERRAT and a high quality 3D structure of the vaccine protein was provided. The physicochemical properties and potential for expression in E.coli was evaluated using many servers. Its interaction with TLR-4 evaluated with cluspro docking.Conclusions: The vaccine is expected to have a high potential to induce in cellular immune responses, high quality structure and suitable properties including high stability, solubility and a high potential expresseion in E.coli. Therefore, this vaccine may be considered as an anti- Brucella abortus vaccine.

کلیدواژه ها:

Bioinformatic Vaccine ، Brucella abortus ، Tertiary structure analysis ، in silico cloning