Evaluation of changes in enzymes of MDA, GPX, SOD, catalase and hepatocytes of male offspring of vitamin E treated rats

Objectives: Vitamin E can provide a good antioxidant, according to scientific evidence regarding the pharmacology and medicinal properties of the liver at different doses. Vitamin E can play an important role in healing liver damage, so we evaluated the extent of changes in liver antioxidant enzymes and liver tissue.materials and methods: Adult male and female Wistar rats weighing 220 20 20 g were used, divided into six experimental groups, and received oral gavage from day zero of gestation until 28 days after birth. And a number of offspring were anesthetized on day 28 after birth and liver was removed for histopathological evaluation and activity of antioxidant enzymes, MDA, GPX, SOD, catalase assay and stored at -80 ° C. Microtomes were cut to about 5 microns in thickness and stained by H&E method and studied by light microscopy (magnification 400). Data were analyzed by SPSS software at significant level (P <0.05).Results: In this study, oxidative enzymes showed a significant decrease in MDA in vitamin E groups compared to the control group (P <0.05) and GPX and catalase levels also increased significantly (P <0.05). And in histopathological studies, the number of hepatocytes in the doses of 100, 200 and 400 vitamin E significantly increased compared to control, NAVE and oil groups (P <0.05).Conclusion: Vitamin E has a beneficial effect on oxidative stress and reduces liver cell toxicity. It also reduces the damage of liver cells, lobules and hepatocytes.

کلیدواژه ها:

Liver ، Vitamin E ، Antioxidant ، Rat ، Histopathology