Evaluation of the properties of the wound dressing film of chitosan / polyvinyl alcohol-based containing human adipose-derived stem cell exosomes, Silver nanoparticles and sildenafil citrate

Objective: Burn wounds are bad prognosis wounds. These wounds have a lot of discharge and regular dressings are not very useful for dressing these wounds. Hydrogels, with plenty of water and the ability to exchange materials, are the best choice for dressing for these wounds. Exosomes as extracellular vesicles play an important role in intercellular signaling and stimulating cell growth Recent studies have shown that sildenafil citrate plays an important role in angiogenesis and wound healing. In this study, we succeeded in making a new hydrogel dressing by combining many findings on wound healing as well as the use of regenerative medicine.Materials and Methods: In this study, briefly, First, several different compounds were made from chitosan-PVA and their properties were investigated and the optimum composition was determined. FTIR, contact angle, tensile strength Biodegradability, Water absorption, and Water vapor passage test has done. Silver nanoparticles were synthesized from silver nitrate and added to the compound. Exosomes Extracted from Human Adipose-derived Stem Cells and Characterized. The raw material of Viagra (sildenafil citrate) was obtained from drug companies and made a 10% by weight suspension. Then dressing with different compounds was placed on 4 groups of Wistar rats then Tissue samples were examined by H&E, Silver and Masson's trichrome stainingResults: Optimal dressing ratio was found. Silver nanoparticle dressing had better antibacterial properties. Real Time pcr for nanog and oct4 genes confirmed that the cells extracted from tissue are stem cell. Western blot analysis confirmed the existence of exosomal markers. A new method was discovered to prepare exosomes for TEM imaging and the rats recovered well. The results in the dressing group showed marked improvement.Conclusion: This combination of tissue engineering, stem cells, nano technology has opened a window of hope to patients with burns bright results were observed in wound healing.