Graphene quantum dots (GQDs) and Fibrin Hydrogel Scaffold Can Induce Osteogenic Differentiation in Wharton’s Jelly derived Mesenchymal Stem Cells

Background and aim: Bone tissue engineering has a progressive role in bone regenerative medicine. Osteogenesis induction is an important process in bone regeneration. Fibrin as a bio- polymeric material and graphene quantum dot (GQD) as a induced differentiation factor have been used as a common material in bone tissue engineering researches. The current study, evaluated the effects of fibrin and GQD on the osteogenic potential of WJ-MSCs.Materials and methods: Third passage WJ-MSCs were cultured on fibrin hydrogel scaffold in DMEM supplemented with 10% FBS and antibiotic in the presence and absence of 10 μg/ml GQD. The culture dishes were incubated 14 days under common cell culture conditions. Their medium changed every 3 days. At the end of the period, in vitro osteogenic potential of cells was determined by 2 common osteogenic marker tests included alkaline phosphatase (ALP) activity and alizarin red staining.Results: Mineralization was occurred within the two - 3D medium groups diagnosed as red colored regions, while, the number of red colored region in GQD group was higher than the control group. Mean ± SEM of ALP enzyme was 207.23± 18.76 and 178.74 ± 2.31 in GQD and control group, respectively however their difference was not significant (P<0.05).Conclusion: In absence of osteogenic differentiating, fibrin and GQD could induce osteogenic differentiation in WJ-MSCs. These elements can enhance osteogenic differentiation potential of MSCs.

کلیدواژه ها:

Fibrin ، GQD ، Scaffold ، WJ-MSC ، Osteogenic ، ALP